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. Author manuscript; available in PMC: 2018 Oct 6.
Published in final edited form as: Org Lett. 2017 Sep 12;19(19):5010–5013. doi: 10.1021/acs.orglett.7b01611

Figure 3.

Figure 3

HPLC analysis of metabolites of various L. enzymogenes strains: (i) ledPHSAF, strain HW with PHSAF being inserted upstream of ledD; (ii) ledPHSAFΔledD, strain ledPHSAF with ledD being in-frame deleted; (iii) ledPHSAFΔledE, strain ledPHSAF with ledE being in-frame deleted; (iv) ledPHSAFΔledF, strain ledPHSAF with ledF being in-frame deleted; (v) ledPHSAFΔledDE, strain ledPHSAF with both ledD & ledE being in-frame deleted; (vi) ledPHSAFΔledEF, strain ledPHSAF with both ledE & ledF being in-frame deleted; (vii) ledPHSAF-ledE-G455E, strain ledPHSAF with ledE A4-domain active site being point-mutated, G455E; (viii) ledPHSAF-ledE-H785V, strain ledPHSAF with ledE C5-domain active site being point-mutated, H785V. (ix) ledPHSAF-ledF-G963E, strain ledPHSAF with ledF A6-domain active site being point-mutated, G963E. (x) ledPHSAF-ledF-H205V, strain ledPHSAF with ledF C6-domain active site being point-mutated, H205V. (xi) ledPHSAF-ledF-Y1357F, strain ledPHSAF with ledF R2-domain active site being point-mutated, Y1357F. The metabolites were detected at UV 315 nm.