Figure 1:

Overview of the experimental setup, sample collection, and data organization. (A) Pups were bilaterally spared or deprived of their C-row whiskers between P12 and P23-P26. Whisker deprivation, i.e., plucking, was repeated every third day to ensure that there was no regrowth of the whiskers. (B) Proteins were denatured and purified, followed by on-filter digestion into tryptic peptides, which were subsequently desalted on C18 StageTips and sequenced on a mass spectrometer. (C) Organization of data files in the database. Colours correspond to the colour code codes in Figs 2, 3, and 5, as well as the MS output file in the Supplementary Data. Sample codes of 5 digits (e.g., A2.1.1.2) indicate a technical replicate of the sample listed above it (e.g., A2.1.1). See Supplementary Table S1 for mapping of samples to mouse IDs.