Table 3:
Experimental | Number of raw | Percentage of QC | Percentage DNA barcode | Percentage OTU clustered |
---|---|---|---|---|
mixture | reads | (pseudo-)readsa | assigned (pseudo-)readsa | (pseudo-)readsa |
EM1 | 466 108 | 88.07 | 95.68 | 83.86 |
EM2 | 448 428 | 86.04 | 97.24 | 84.04 |
EM3 | 496 328 | 87.46 | 96.61 | 84.34 |
EM4 | 273 104 | 77.38 | 95.74 | 80.54 |
EM5 | 582 254 | 96.26 | 97.84 | 90.63 |
EM6 | 442 574 | 92.81 | 97.54 | 81.48 |
EM7 | 394 354 | 93.04 | 97.14 | 80.70 |
EM8 | 455 172 | 79.62 | 95.66 | 82.35 |
EM9 | 434 326 | 86.23 | 97.30 | 83.60 |
EM10 | 387 816 | 87.73 | 97.00 | 75.11 |
EM10R | 723 130 | 95.59 | 98.02 | 87.39 |
EM11 | 363 374 | 84.44 | 96.74 | 78.63 |
EM11R | 635 304 | 91.11 | 98.21 | 87.01 |
EM12 | 355 634 | 92.55 | 97.54 | 76.54 |
EM13 | 405 742 | 89.46 | 96.49 | 77.31 |
EM14 | 480 772 | 85.74 | 95.98 | 81.91 |
EM15 | 554 602 | 87.05 | 88.78 | 82.98 |
Averageb | 464 648 | 88.27 | 96.44 | 82.26 |
a(Pseudo-)reads are the combined QC pseudo-reads and the QC processed unmerged forward and reverse reads.
bAveraged across the 17 Illumina MiSeq datasets.