Table 1:
Sequencing libraries and data yields for whole-genome shotgun sequencing
| Library type | Lane | Read length (bp) | Insert size (bp) | Raw bases | Clean bases | ||
|---|---|---|---|---|---|---|---|
| Total bases (Gb) | Depth (×) | Total bases (Gb) | Depth (×) | ||||
| PE101 | 2 | 100 | 170 | 80.47 | 115.30 | 74.12 | 106.20 |
| PE151 | 1 | 150 | 250 | 59.69 | 85.52 | 47.20 | 67.63 |
| PE101 | 4 | 100 | 500 | 47.89 | 68.62 | 43.58 | 62.44 |
| PE101 | 3 | 100 | 800 | 42.22 | 60.49 | 36.79 | 52.71 |
| MP50 | 2 | 49 | 2000 | 30.36 | 43.50 | 19.56 | 28.03 |
| MP50 | 3 | 49 | 5000 | 23.11 | 33.11 | 9.06 | 12.98 |
| MP50 | 3 | 49 | 10 000 | 20.17 | 28.90 | 6.71 | 9.61 |
| MP50 | 2 | 49 | 20 000 | 19.01 | 27.24 | 4.35 | 6.23 |
| MP50 | 1 | 49 | 40 000 | 13.91 | 19.93 | 4.69 | 6.72 |
| Total | 21 | 336.83 | 482.61 | 246.06 | 352.55 | ||
Sequencing depth was calculated based on a genome size of 697.94 Mb. High-quality data were obtained by filtering raw data for low-quality and duplicate reads.