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. 2017 Sep;46(9):1204–1210.

Table 1:

Methylated and unmethylated of p16INK4α gene primer sequences.

Primer name Primer sequence (5′ – 3′) X°C P
p16 -M-F TTATTAGAGGGTGGGGCGGATCGC 63°C 149
p16 -M-R GACCCCGAACCGCGACCGTAA
p16 -U-F TTATTAGAGGGTGGGGTGGATTGT 56°C 150
p16 -U-R CAACCCCAAACCACAACCATAA
*

Note: CpG islands were bold and underlined; X°C: primer annealing temperature. M: methylated, U: Unmethylated; F: Forward; R: Reverse; P: product size.