Fig. 2.

Thymoquinone inhibits NADPH oxidase-mediated ROS generation in LPS-activated BV2 microglia. a BV2 cells were treated with vehicle or thymoquinone (2.5–10 µM) for 30 min prior to LPS stimulation for 24 h. ROS generation was measured in live cells by the fluorescence detection of dichlorofluorescein. (Mean ± SEM; ***p < 0.001; one-way ANOVA with post hoc Student Newman–Keuls test). b BV2 cells were treated with vehicle or thymoquinone (2.5–10 µM) for 30 min prior to LPS stimulation for 24 h. Cell lysates were analysed using immunoblotting for p-p40^phox and actin. Representative blots and densitometric analyses of three independent experiments are shown (Mean ± SEM; **p < 0.01; one-way ANOVA with post hoc Student Newman–Keuls test). c BV2 cells were treated with vehicle or thymoquinone (2.5–10 µM) for 30 min prior to LPS stimulation for 24 h. Membrane extracts were analysed using immunoblotting for gp91^phox and actin. Representative blots and densitometric analyses of three independent experiments are shown (Mean ± SEM; **p < 0.01, ***p < 0.001; one-way with ANOVA post hoc Student Newman–Keuls test)