Figure 5.

Activation of IFN regulatory factor 5 (IRF5) by TLR8 ligand and Group B Streptococcus (GBS) is antagonized by TLR2 costimulation, while activation of IRF5 by viable Escherichia coli is not. Monocytes were stimulated with CL75 (1 μg/ml), viable or heat-inactivated (HI) GBS (MOI 1.0) and E. coli (MOI 2.0), and with or without FSL-1 costimulation (100 ng/ml) for 2 h. The monocytes were subsequently fixed and double IF stained IRF5 and p65/RelA. Nuclear accumulation was quantified by high-content screening (Scan^R, 20×). NS, no stimuli. nd, not done. n = 4.