Table 3. Analysis of DE genes with known or suspected roles in honey bee reproduction or related process.
| Gene id | Gene name | Correlate | Ref. | Expression level in this study | ||
|---|---|---|---|---|---|---|
| Q vs. V | C vs. Q | R vs. C | ||||
| 408961 | Apolipophorins (known as RFABP) | JH response genes | Pandey & Bloch (2015) | Down-regulated | Up-regulated | Down-regulated |
| 552515 | ATP-dependent RNA helicase WM6 (known as Helicase at 25E) | Higher expressed in ovaries of queen larvae compared with worker larvae in fourth and early fifth larvae | Lago et al. (2016) | Up-regulated | Down-regulated but not significantly | Up-regulated but not significantly |
| 408827 | Carbonic anhydrase 1 (CAH1) | JH response genes | Pandey & Bloch (2015) | Down-regulated | Up-regulated but not significantly | Down-regulated but not significantly |
| 413762 | Complement component 1 Q subcomponent-binding protein, mitochondrial | Higher expressed in ovaries of queen larvae compared with worker larvae in fourth and early fifth larvae | Lago et al. (2016) | Down-regulated | Down-regulated but not significantly | Up-regulated |
| 726690 | Cytochrome P450 6AS3 (CYP6AS3) | Up-regulated in the EcR knock down bees | Mello et al. (2014) | Down-regulated | Up-regulated | Down-regulated but not significantly |
| 412209 | Cytochrome P450 6AS4 (CYP6AS4) | Up-regulated in the EcR knock down bees | Mello et al. (2014) | Down-regulated | Up-regulated | Down-regulated |
| 409677 | Cytochrome P450 6AS5 (CYP6AS5) | Up-regulated in the EcR knock down bees | Mello et al. (2014) | Down-regulated | Up-regulated | Down-regulated |
| 551560 | Cytochrome P450 6BD1 (CYP6BD1) | Up-regulated in the EcR knock down bees | Mello et al. (2014) | Down-regulated | Up-regulated | Down-regulated |
| 411057 | Cytochrome P450 314 A1 (CYP314A1) | coded for Ec dysone 20-hydroxylase | Rewitz et al. (2006) | Up-regulated | No change | Down-regulated but not significantly |
| 406143 | Defensin 1 (Def1) | Up-regulated in mated queens compared with virgin queens | Manfredini et al. (2015) | Down-regulated | Up-regulated | Down-regulated |
| 406070 | Dopamine receptor 2 (Dopr2) | Ec response genes | Rewitz et al. (2006) | Down-regulated | No change | Up-regulated |
| 410309 | Ecdysone-induced protein 75 (E75) | Ec response genes | Rewitz et al. (2006) | Up-regulated | Up-regulated but not significantly | No change |
| 406084 | Ecdysone receptor (EcR) | Ec response genes | Rewitz et al. (2006) | Up-regulated | Not detected | Not detected |
| 408758 | Ecdysteroid-regulated gene E74 (E74) | Ec response genes | Rewitz et al. (2006) | Up-regulated | No change | No change |
| 409384 | Heat shock protein 60 (Hsp60) | Higher expressed in ovaries of queen larvae compared with worker larvae in fourth and early fifth larvae | Lago et al. (2016) | Up-regulated but not significantly | Down-regulated | Up-regulated |
| 408928 | Heat shock protein 90 (Hsp90) | Higher expressed in ovaries of queen larvae compared with worker larvae in fourth and early fifth larvae; candidate marker genes for caste-specific ovary development; | Lago et al. (2016) | Up-regulated | Down-regulated | Up-regulated |
| 408818 | Hexokinase (HK) | QMP response genes | Hoover et al. (2003) | Up-regulated | No change | Down-regulated but not significantly |
| 406117 | Hexamerin 70b (Hex70b) | JH response gene, highly expressed in fourth and early fifth-instar queen ovaries | Lago et al. (2016) | Up-regulated | Up-regulated but not significantly | Down-regulated but not significantly |
| 726542 | Histone H3 | QMP response genes; overrepresented in ovaries of queens in the fifth larval instar | Humann & Hartfelder (2011) | Down-regulated | No change | Down-regulated but not significantly |
| 102655073 | Histone H4 | QMP response genes | Hoover et al. (2003) | Up-regulated | Up-regulated but not significantly | Down-regulated but not significantly |
| 726965 | JH-inducible protein | JH and Ec response gene, up-regulated in Ec knock down bess | Mello et al. (2014) | Up-regulated | Down-regulated but not significantly | No change |
| 100576395 | Kruppel homolog 1 (Kr-h1) | An immediate response gene in the JH response cascade | Lago et al. (2016) | Up-regulated | Down-regulated but not significantly | Up-regulated but not significantly |
| 406121 | Major royal jelly protein 3 (Mrjp3) | Ec response gene; down-regulated in Ec knock down bees | Mello et al. (2014) | Down-regulated | Up-regulated but not significantly | Down-regulated but not significantly |
| 409870 | Minor histocompatibility antigen H13 | Higher expressed in ovaries of queen larvae compared with worker larvae in fourth and early fifth larvae | Lago et al. (2016) | Up-regulated | Down-regulated but not significantly | No change |
| 411820 | Mitogen-activated protein kinase phosphatase-3 (Mapk-3) | Higher expressed in ovaries of queen larvae compared with worker larvae in fourth and early fifth larvae | Lago et al. (2016) | Down-regulated | Up-regulated but not significantly | Up-regulated but not significantly |
| 408572 | Myophilin (CHD64) | JH response genes | Rewitz et al. (2006) | Down-regulated | Down-regulated but not significantly | Up-regulated but not significantly |
| 409881 | Myosin regulatory light chain 2 (Mlc2) | Up-regulated in mated queens compared with virgin queens | Manfredini et al. (2015) | Down-regulated | Down-regulated | Up-regulated but not significantly |
| 552193 | Proton-coupled amino acid transporter | QMP response genes | Hoover et al. (2003) | Up-regulated | No change | Down-regulated but not significantly |
| 409681 | RWD domain-containing protein 1 (RWDD1) | QMP response genes | Hoover et al. (2003) | Up-regulated | Down-regulated but not significantly | Down-regulated but not significantly |
| 409227 | Ultraspiracle (USP) | Ec and JH response genes | Rewitz et al. (2006) | Up-regulated but not significantly | Down-regulated but not significantly | Up-regulated |
| 406088 | Vitellogenin (Vg) | The protein product serves as a yolk precursor in egg development | Nunes et al. (2013) | Down-regulated | Up-regulated | Down-regulated |
Notes.
- V
- group of virgin queens (n = 3)
- Q
- group of egg-laying queens (n = 3)
- C
- group of egg-laying inhibited queens (n = 3)
- R
- group of egg-laying recovery queens (n = 3)