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. 2017 Sep 27;8:1673. doi: 10.3389/fpls.2017.01673

FIGURE 1.

FIGURE 1

Fast and cost-effective ShortPhos phosphoproteomic workflow with single run analysis of phosphoproteome compared with conventional workflow. (A) An overview of the general procedure of optimized MF isolation method. (B) Conventional phosphoproteomics workflow usually requires 500–4000 μg of plasma membrane (PM) for protein digestion, peptides desalting, peptides fractionation, phosphopeptides enrichment by TiO2 or IMAC and multiple LC-MS/MS runs per sample, resulting in slow and costly workflow in large scale experiment. (C) “ShortPhos” phosphoproteomic workflow described in this paper requires minimal microsomal fraction (MF) for protein digestion, no desalting or fractionation before phosphopeptide enrichment, and a single run of LC-MS/MS analysis per sample.