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. 2017 Oct 4;8:1683. doi: 10.3389/fpls.2017.01683

FIGURE 1.

FIGURE 1

Tr-KPI transcript abundance is responsive to water deficit treatment in white clover. Transcription of Tr-KPI1, Tr-KPI2, and Tr-KPI5 in the first fully expanded leaf (FFE) of white clover ecotype Tienshan (A) and cultivar Kopu (B), as indicated, under NPS and PS treatments. Relative transcript abundance was determined by qRT-PCR using two biological replicates, with pooled tissues collected from at least three individual plants comprising a biological replicate, and was normalized using two internal reference genes, Tr-β-actin and Tr-GAPDH. The moisture content represent the mean value of the biological replicates and each data point represents mean value ± SE, of the biological replicates. Statistical analysis was performed using Student’s t-test. ∗Indicate statistically significant (P < 0.05) differential expression in comparison with initial moisture content for NPS and PS treatment, respectively, and ‘†’ indicates statistically significant (P < 0.05) expression in the PS treatment in comparison with the NPS treatments using Student’s t-test.