FIGURE 2.

Tr-KPI transcript abundance is responsive to water deficit treatment in white clover cultivar ‘Huia.’ Relative transcript abundance of Tr-KPI1 (A), Tr-KPI2 (B), and Tr-KPI5 (C) in the first fully expanded leaf (FFE) of white clover, as indicated, under NPS and PS treatments. Relative transcript abundance was determined by qRT-PCR using at least three biological replicates, with pooled tissues collected from at least three stolons comprising a biological replicate, and was normalized using two internal reference genes, Tr-β-actin and Tr-GAPDH. The moisture content represent the mean value of the biological replicates and each data point represents mean value ± SE, of the biological replicates. Statistical analysis was performed using Student’s t-test. ^∗Indicate statistically significant (P < 0.05) differential expression with the initial moisture content of the NPS or PS treatment, respectively. ^†Indicates statistically significant (P < 0.05) differential expression in the PS in comparison with the NPS treatments using Student’s t-test.