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. 2017 Oct 4;8:1683. doi: 10.3389/fpls.2017.01683

FIGURE 5.

FIGURE 5

Changes in the transcript abundance of ET biosynthesis genes in the RNAi lines. Expression of the genes encoding ACC- synthase (Tr-ACS1) (A) and ACC-oxidase1(Tr-ACO1) (B) and ACC-oxidase 2 (Tr-ACO2) (C) in the first fully expanded leaf (FFE) of white clover RNAi lines in response to PS treatment against the moisture content of the media, as indicated. Relative transcript abundance was determined by qRT-PCR using two biological replicates, with pooled tissues collected from three genetically independent lines comprising a biological replicate, and was normalized using two internal reference genes, Tr-β-actin and Tr-GAPDH. Each data point represents mean value ± SE of the biological replicates (n = 2). Statistical analysis was performed using Student’s t-test. ∗Indicates statistically significant (P < 0.05) differential expression in comparison with initial moisture content. †Indicates statistically significant (P < 0.05) expression in the PS treatment in comparison with control group using Student’s t-test.