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. 2017 Oct 4;8:1877. doi: 10.3389/fmicb.2017.01877

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Copyright © 2017 Takano, Seki, Kim, Kilgore, Fuwa, Takahashi, Inazaki and Hayakawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visual inspection and dye-mediated monitoring of the H. influenzae serotype-specific LAMP assay. (A), Detection of LAMP reaction using a colorimetric dye (leuco triphenylmethane; D-QUICK). The results were easily recognized by the naked eye, without UV-lamp. (B), Results of a non-Hib LAMP assay (H. influenzae type a) using D-QUICK plus MyAbscope^®. Numbers of bacteria decrease from left to right in the figure (100,000 to 1 copy number of DNA per reaction). (C), Linear regression curve of LAMP results (H. influenzae type a) obtained using D-QUICK plus MyAbscope^®.