Fig. 4.

Effect of microtopography on matrix calcification. (A) The cells were cultured on microtopographic surfaces for 3 weeks in osteoblastogenic medium. They were then fixed and stained for deposition calcified matrix with Alizarin Red (red). Scalebar=1 mm. (B) Percent area of positively stained calcified matrix was quantified with ImageJ. Error bars represent mean±standard error (n=4). *significantly different from control, p<0.05. A table with p-values between significantly different patterns is provided in Supplementary Data.