Figure 5.

Differential expression analysis of miRNA in caryopses under drought stress. (A) Expression profile of differentially expressed miRNA by RT-PCR. CK indicates control condition and DS indicates drought stress. (B,F) TPM value and relative expression level of tae-nsmR10; (C,G) TPM value and relative expression level of tae-csmR5082-1; (D,H) TPM value and relative expression level of tae-nsmR5/6; (E,I) TPM value and relative expression level of tae-miR9654a-3p. Each miRNA expression value was normalized against the internal control gene U6. Relative expression levels were presented as mean ± SE, and means were calculated from three replicates. Asterisks between the control and drought stress indicate significant difference at p < 0.05 as determined by t-test.