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. 2017 Oct 4;8:1693. doi: 10.3389/fpls.2017.01693

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Copyright © 2017 Ali, Mir, Tyagi, Mehari, Meena, Bhat, Yadav, Papalou, Rawat and Grover.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of cell death and fungal biomass in BjNPR1 transgenic and wild-type plants using trypan blue staining. (A) Microscopic examination of Alternaria mediated cell death in BjNPR1 lines and wild-type plants are shown with white color arrows. (B) Trypan blue staining of BjNPR1 transgenic and control plants after Alternaria infection. Alternaria spore load or biomass in BjNPR1 transgenic lines and wild-type plants at various dpi are shown with black color arrows. BjNPR1 transgenic lines showed reduced cell death and spore count as compared to wild-type plants. Bar = 40 μm.