Fig. 5.

Intestinal and systemic nitric oxide secretion following peroral multidrug-resistant P. aeruginosa association of secondary abiotic IL10^–/– mice lacking TLR4. Secondary abiotic IL10-deficient (IL10^–/–; circles) and TLR4-deficient IL10^–/– mice (TLR4^–/– × IL10^–/–; triangles) were perorally challenged with a clinical multidrug-resistant P. aeruginosa strain (PA; closed symbols). Two weeks thereafter, nitric oxide concentrations were measured in supernatants of ex vivo biopsies derived from (A) colon, (B) ileum, (C) mesenteric lymph nodes (MLN), and (D) spleen. Non-infected mice (N; open symbols) served as negative controls. Numbers of mice (in parentheses), means (black bars), and significance levels (p values) determined by the Mann–Whitney U test are indicated. Data shown were pooled from two independent experiments