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. Author manuscript; available in PMC: 2017 Oct 9.
Published in final edited form as: ACS Infect Dis. 2017 Mar 21;3(5):336–348. doi: 10.1021/acsinfecdis.6b00179

Figure 1. Crosslinker-dependent higher molecular-weight adducts form only in the when LprG is expressed in the cell wall.

Figure 1

Msm expressing C-terminally His-tagged LprG or the soluble truncated form NA-LprG were cultured and split into two samples, one for DSP treatment and the other for DMSO-vehicle control. A portion of the LprG sample was also treated with the reducing agent dithiothreitol (DTT) to cleave the internal disulfide of DSP.