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. Author manuscript; available in PMC: 2017 Oct 9.
Published in final edited form as: ACS Infect Dis. 2017 Mar 21;3(5):336–348. doi: 10.1021/acsinfecdis.6b00179

Figure 4. Optimization of irradiation conditions for site-specific crosslinking.

Figure 4

Msm expressing LprG(Y3xH) or LprG(98pBpa) were grown with 2 mM pBpa and live cells were UV-irradiated for 0, 5, 10, 20 or 30 min. Total lysates were probed for FLAG-tagged LprG by SDS-PAGE and immunoblot.