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. Author manuscript; available in PMC: 2017 Oct 9.
Published in final edited form as: ACS Infect Dis. 2017 Mar 21;3(5):336–348. doi: 10.1021/acsinfecdis.6b00179

Figure 5.

Figure 5

Photocrosslinking of mutants containing pBpa identifies multiple distinct LprG interaction interfaces by immunoblot analysis. Msm harboring the indicated LprG(pBpa) mutants of FLAG-tagged LprG were grown with pBpa, and UV-irradiated for 0 or 30 min. Total lysates were probed for LprG by α-FLAG immunoblot. Arrowheads indicate examples of prominent UV-dependent higher molecular-weight adducts. Mutants are grouped by their relative location within Msm LprG. Data are representative of at least 2 biological replicates.