FIGURE 6.

Expression of CD7 and CD127 induced in HPC subpopulations by Notch signaling. (A) Generation of CD34⁺Lin⁻CD7⁺ and CD34^low/−Lin⁻CD7⁺ cells from HPC-1, HPC-2, and HPC-3 cells in culture with IL-7 in the presence and absence of Notch signaling for 2 wk. In this experiment, the CD7⁻ fraction of each HPC subpopulation was sorted to exclude the possible expansion of endogenous CD34⁺CD7⁺ cells. T, B, and myeloid lineage cells were gated out by using CD3, CD11c, CD14, CD15, CD19, and CD20 Abs for detection of CD34⁺Lin⁻CD7⁺ cells and CD34^low/−Lin⁻CD7⁺ cells. (B) Time course for the generation of CD34⁺Lin⁻CD7⁺ and CD34^low/−Lin⁻CD7⁺ cells in association with CD127 expression from the total HPC population (upper) and HPC subpopulation (lower) under (+) or (−)NL/(−)IL-7(−)IL-15 conditions. Six color analyses using CD5, CD7, CD34, CD56, CD127, and lineage (CD3, CD11c, CD14, CD15, CD19, and CD20) were conducted. CD127 expression in the CD34^low/− cell populations from the three HPC subpopulations is shown at the week when each subpopulation started to express CD127 (lower). CD5 and CD56 expressions in CD34^low/−Lin⁻CD7⁺CD127⁺cells were analyzed for HPC subpopulations. Similar results were obtained from two experiments for the other two donors.