Table 1.

Comparison of the LoD in terms of means, standard deviation, coefficient of variation percentage as a measure of accuracy of the 6 species of Leishmania for each molecular marker by qPCR.

Marker	Species	10-3 (0,001 parasite equivalents/mL)			10-2 (0.01 parasite equivalents/mL)			10-1 (0.1) parasite equivalents/mL			100 (1 parasite equivalent/mL)
		Mean (Ct)	SD	CV%	Mean (Ct)	SD	CV%	Mean (Ct)	SD	CV%	Mean (Ct)	SD	CV%
HSP70	L. amazonensis				33.32	1.76	5.29	29.87	0.95	3.19	27.45	0.37	1.34
	L. braziliensis				33.29	1.60	4.82	30.09	0.74	2.44	27.56	0.47	1.69
	L. guyanensis				31.88	1.79	5.60	29.92	1.01	3.36	27.5	0.52	1.88
	L. panamensis				31.75	2.66	8.37	30.26	0.86	2.83	27.59	0.43	1.55
	L. mexicana				33.99	1.46	4.29	30.38	0.68	2.24	28.05	0.48	1.71
	L. infantum				34.29	2.19	6.39	30.03	0.63	2.10	27.43	0.45	1.66
ITS	L. amazonensis				36.76	0.84	2.30	34	0.37	1.07	30.14	0.37	1.24
	L. braziliensis				36.56	0.80	2.19	34.22	0.39	1.15	30.07	0.33	1.09
	L. guyanensis				37.58	0.85	2.26	34.43	0.39	1.13	30.14	0.35	1.17
	L. panamensis				37.14	0.79	2.13	33.93	0.41	1.21	30.03	0.47	1.57
	L. mexicana				37.55	0.65	1.74	34.48	0.39	1.14	30.21	0.44	1.46
	L. infantum				36.77	0.75	2.03	34.38	0.43	1.26	29.98	0.36	1.21
kDNA	L. amazonensis	29.57	0.34	1.14	23.83	0.24	0.99	20.3	0.22	1.07
	L. braziliensis	29.85	0.31	1.04	24.52	0.11	0.46	19.46	0.20	1.00
	L. guyanensis	30.1	0.37	1.24	24.67	0.34	1.39	19.44	0.26	1.32
	L. panamensis	29.99	0.45	1.49	24.54	0.26	1.09	20.18	0.21	1.05
	L. mexicana	30.41	0.40	1.33	24.09	0.30	1.24	20.21	0.22	1.11
	L. infantum	29.99	0.40	1.35	24.09	0.31	1.27	19.99	0.21	1.06
18 S	L. amazonensis	39.03	0.63	1.61	34.91	0.45	1.30	31.51	0.43	1.35
	L. braziliensis	38.74	0.56	1.44	35.03	0.37	1.05	31.27	0.39	1.24
	L. guyanensis	38.89	0.78	2.01	34.83	0.46	1.33	31.25	0.58	1.84
	L. panamensis	39.06	0.49	1.24	34.72	0.41	1.17	31.44	0.45	1.44
	L. mexicana	38.67	0.81	2.10	34.77	0.61	1.76	31.32	0.40	1.28
	L. infantum	39.62	0.76	1.92	34.75	0.47	1.36	31.53	0.48	1.51