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. 2017 Sep 25;2017:3781904. doi: 10.1155/2017/3781904

Figure 4.

Figure 4

SIRT1 deficiency inhibited migration of BCa cells. (a) Transwell migration assay was conducted to measure the migration ability of NC group cells (A, C) and si-SIRT1 group cells (B, D) in EJ (contaminated by T24 as per “http://iclac.org/databases/cross-contaminations/”) and T24 for 48 h after transfection. (b) BCa cells in transwell migration assay were counted on microscope and statistically analyzed. All shown values were mean ± SD of three measurements and repeated three times with similar results. ∗p < 0.05; ∗∗p < 0.01. (c) Representative result of immunofluorescence staining of E-cadherin (green) in the BCa cells after SIRT1-target-specific-siRNA treatment (KD) (B, D), compared with control-siRNA treatment (NC) (A, C). Nuclei (blue) were stained by DAPI. The scale bar for (D) is 40 μm. (d) Representative result of immunofluorescence staining of N-cadherin (green) in the BCa cells after SIRT1-target-specific-siRNA treatment (KD) (B, D), compared with control-siRNA treatment (NC) (A, C). Nuclei (blue) were stained by DAPI. The scale bars for (a) are 25 μm and for (c-d) are 40 μm.