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The specific fragmentation process of mangiferin flavonoids, take neomangiferin as an example: The fragment ion at m/z 583 [M-H]− was a molecular ion, m/z 421 displayed a loss of 162 Da (C6H10O5) based on the parent ion, the fragment ion at m/z 331 [M-H-C6H10O5-C3H6O3]− conformed to the rule of C-glycosides loss 90 Da (C3H6O3) and m/z 301 [M-H-C6H10O5-C4H8O4]− followed the rules of C-glycosides loss 120 Da (C4H8O4) from sugar molecules through 0,3X/0,2X cleavage.
