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. 2017 Sep 25;2017:5314649. doi: 10.1155/2017/5314649

Figure 3.

Figure 3

NEAT1 functioned as an endogenous sponge to downregulate miR-129 by competitively binding to miR-129. qRT-PCR was performed to detect the expression of miR-129 in EC109 (a) and EC9706 (b) cells transfected with si-NEAT1 or si-control. (c, e) The predicted binding sites between miR-129 on NEAT1, as well as the mutants of NEAT1 and miR-129. (d) The relative luciferase activity in EC109 and EC9706 cells cotransfected with luciferase reporter vectors containing NEAT1-WT or NEAT1-MUT and miR-control or miR-129. (f) The relative luciferase activity in EC109 and EC9706 cells cotransfected with miR-129-WT or miR-129-MUT reporter and pcDNA or pcDNA-NEAT1. RIP assay was conducted in EC109 (g) and EC9706 (h) cell extracts to examine miR-129 endogenously associated with NEAT1. ∗P < 0.05.