Figure 3.

Resveratrol supplementation does not affect copper-induced expression of senescence-associated molecules. (a) Transcript-relative levels of cyclin-dependent kinase inhibitor 1A (p21), apolipoprotein J (ApoJ), and transforming growth factor beta 1 (TGFβ1) were assessed by qPCR in 350 μM CuSO₄-treated fibroblasts that were allowed to recover in the presence of the indicated doses of resveratrol. (b) Representative western blots of p21 and ApoJ protein levels are depicted; the resulting densitometric analysis, normalized for control cells in the absence of resveratrol, is plotted for each analyzed protein. Ponceau S staining was used to control for protein loading. Data are presented as means ± SEM of at least three independent experiments. ^∗p < 0.05; ^∗∗p < 0.01; and ^nsnonsignificant, for the comparisons between the indicated groups.