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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Mech Ageing Dev. 2017 Aug 19;166:16–23. doi: 10.1016/j.mad.2017.08.007

Figure 4.

Figure 4

Increased aggrecanolysis and ADAMTS4 expression in oxidative stress-induced senescent hNP cell culture. (A) Western analysis of aggrecan in hNP senescent and control conditioned media. MMP- and ADAMTS-mediate cleavage sites within the interglobular domain of aggrecan (top right). Bottom graphs showed quantification of aggrecan fragments using densitometry. The dotted line represents a ratio of 1, indicating that the amount of aggrecan fragments generated by senescent hNP cells is that same as that by nonsenescent hNP cells. The blue square represents the mean. The error bars represent the SEM (n = 6). (B) Immunofluorescence revealed upregulated expression of ADAMTS4 protein (green) in senescent hNP cells.