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Independent variables and experimental design levels in the Box–Behnken design for determining the optimal UAEE condition of crude TFP extraction.
| Independent variables | Coded symbols | Levels | ||
|---|---|---|---|---|
| −1 | 0 | 1 | ||
| pH | X 1 | 4 | 4.5 | 5 |
| Enzyme amount (u/g) | X 2 | 4000 | 5000 | 6000 |
| Extraction temperature (°C) | X 3 | 40 | 45 | 50 |
| Liquid-to-solid ratio (ml/g) | X 4 | 20 | 30 | 40 |
