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. 2017 Oct;58(12):5030–5042. doi: 10.1167/iovs.16-21402

Figure 4.

Figure 4

Regulation of inflammation factors by Y-0452. R28 cells were treated with 25 μmol/L Y-0452 for 4 hours and then co-incubated with 200 μmol/L palmitate (Pal) for another 24 hours. (A) Levels of total NF-κB (t-NF-κB), phosphorylated NF-κB (p-NF-κB), IκBα, and PPARα were determined by Western blotting. β-actin was used as an internal control. Representative blots were shown from three independent experiments. (B–E) Densitometry quantification of t-NF-κB, p-NF-κB, IκBα, and PPARα levels (n = 3, *P<0.05 versus vehicle, 2-way ANOVA).