Figure 2.

TOP1MT silencing enhanced the invasion and migration of BGC-823 and SGC-7901 cells in vitro and in vivo. (A, B and C) Migration and invasion of GC cells infected with TOP1MT siRNA (SiTOP1MT) or negative control siRNA (SiNC) or negative control (NC) were assessed by transwell migration assays (A), Boyden chamber invasion transwell assays (B), and wound closure assays (C). (D) BGC-823/scramble, BGC-823/shTOP1MT, SGC-7901/scramble, and SGC-7901/shTOP1MT were subcutaneously injected into the flanks of female athymic mice. The volumes (D, upper) of the xenotransplanted tumors were calculated every 4 days. Representative images of subcutaneous xenotransplanted tumors (D, lower left) and tumor weights (D, lower right) were measured when the mice were killed on day 28 post implantation. (E) Gross lung metastases (E, left) and lung metastases stained with hematoxylin and eosin (HE staining) (E, right) for BGC-823 and SGC-7901 cells. *P < 0.05 in comparisons of the SiTOP1MT-transfected group with the SiNC or NC group. A full colour version of this figure is available at http://dx.doi.org/10.1530/ERC-17-0058.