Table 4.
Real-time PCR primers for mRNAs
h is human; m is mouse. FW is forward, and RE is reverse.
| Primer name | Primer sequence | Primer name | Primer sequence |
|---|---|---|---|
| hCOL1A1-FW | CGAAGACATCCCACCAATCAC | hWNT7b-FW | GCGTTACGGCATCGACTTCT |
| hCOL1A1-RE | CAGATCACGTCATCGCACAAC | hWNT7b-RE | GCTGCATCCGGTCCTCTAGA |
| hCOL3A1-FW | TGGCTACTTCTCGCTCTGCTT | hWNT9a-FW | GCAGCAAGTTCGTCAAGGAAT |
| hCOL3A1-RE | TTCCAGACATCTCTATCCGCATAG | hWNT9a-RE | GTCTCCACCCCAGCCTTGAT |
| hCOL4A1-FW | CTCTGGCTGTGGCAAATGTG | hWNT1-FW | CAAGTGGCAGTTCCGGAATC |
| hCOL4A1-RE | CCTCAGGTCCTTGCATTCCA | hWNT1-RE | GCGGAGGTGATAGCGAAGATA |
| hASMA-FW | GTGTTGCCCCTGAAGAGCAT | hNFATC2-FW | CGGGCCCACTATGAGACAGA |
| hASMA-RE | CGCCTGGATAGCCACATACAT | hNFATC2-RE | CCAGAGGCTTGTTTTCCATGTAG |
| hGAPDH-FW | GAAGGTGAAGGTCGGAGTCAAC | hTGFBR1-FW | GGTTCCGTGAGGCAGAGATTTA |
| hGAPDH-RE | CATGGGTGGAATCATATTGGAA | hTGFBR1-RE | CTGACACCAACCAGAGCTGAGT |
| hFZD4-FW | TTTCACACCGCTCATCCAGTAC | hETS1-FW | GGACCGTGCTGACCTCAATAA |
| hFZD4-RE | GGGTTCACAGCTCTCTTGACT | hETS1-RE | TTCCAGAAGAAACTGCCATAGCT |
| hFZD6-FW | CGGATGTTACGTCTATGAGCAAGT | hETS2-FW | CAGCGGCAGGATGAATGATT |
| hFZD6-RE | CTGGTCGAGCTTTTGCTTTTG | hETS2-RE | GACCCATCAAAGGTGTCAAAGG |
| hEZH2-FW | TCCTACATCCTTTTCATGCAACAC | mCOL1A1-FW | ACGCATGGCCAAGAAGACAT |
| hEZH2-RE | CCCTCCAAATGCTGGTAACAC | mCOL1A1-RE | TTGTGGCAGATACAGATCAAGCA |
| hWNT11-FW | AAGGACTCGGAACTCGTCTATCTG | mCOL3A1-FW | CACCCTTCTTCATCCCACTCTT |
| hWNT11-RE | CCGTTGGATGTCTTGTTGCA | mCOL3A1-RE | TGACATGGTTCTGGCTTCCA |
| hWNT2-FW | TGGCTCCCTCTGCTCTTGAC | mGAPDH-F | CTCGTCCCGTAGACAAAATGGT |
| hWNT2-RE | TGGCACATTATCGCACATCAC | mGAPDH-R | TGATGGCAACAATCTCCACTTT |
| hWNT3a-FW | ATGGCCCCACTCGGATACTT | mNFATC2-FW | GAACTTCACAGCGGAGTCCAA |
| hWNT3a-RE | GAGGAATACTGTGGCCCAACA | mNFATC2-RE | GCATGTTAGGCTGGCTCTTGT |
| hWNT4-FW | CTCGTCTTCGCCGTCTTCTC | mETS1-FW | CGACTCTCACCATCATCAAGACA |
| hWNT4-RE | GGATCAGGCCCTTGAGTTTCT | mETS1-RE | TCTTTGCTGCTCGGAGTTAACA |
| hWNT5a-FW | GAGTGCTCGCATCCTCATGA | mETS2-FW | CGCGATGAATGACTTTGGAAT |
| hWNT5a-RE | CTGCAGCCAGCATGTCTTCA | mETS2-RE | GCCATCGAAGGTGTCAAAGG |
| hWNT5b-FW | AGGAATGCCAGCACCAGTTC | mFZD4-FW | CCAGCCAGCTGCAGTTCTTC |
| hWNT5b-RE | GGTCTCTCGGCTGCCTATCTG | mFZD4-RE | TCACAGCGTCTCTTGACTGAAAG |
| hSMAD2-FW | AAGCCATCACCACTCAAAACTGT | mFZD6-FW | CCCTAACCTGATGGGTCATTATG |
| hSMAD2-RE | GGCCTGTTGTATCCCACTGATC | mFZD6-RE | AGCTTGGCAAAGGAACATTTCA |
| hSMAD3-FW | CCACCAGATGAACCACAGCAT | mTGFBR1-FW | GCAATCAGGACCACTGCAATAA |
| hSMAD3-RE | CCGGCTCGCAGTAGGTAACT | mTGFBR1-RE | GCAGACTGGACCAGCAATGAC |