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. 2017 Aug 10;292(40):16665–16676. doi: 10.1074/jbc.M117.803320

Figure 5.

Figure 5.

The pro-part of proNGF perturbs the conformation of the mature part of proNGF. Absolute deuterium incorporation is plotted as a function of time for NGF (gray lines) and the mature part of proNGF (red lines). Equilibrium-labeled (90%) proNGF (control samples) are plotted as blue-filled circles at the 16-h time point. Regions in the mature part of proNGF where a significant protection of exchange was observed in the presence of the pro-part for at least two consecutive time points are colored red on a crystal structure of NGF (PDB ID: 1SG1). S.D. is plotted as error bars (most are too small to be visible). Asterisk, denotes a significant protection from exchange when comparing deuterium incorporation in NGF with the mature part of proNGF (p < 0.01). (n = 3 for the 15-s, 1-min, and 60-min time points, and n = 2 for the 16-h time point and the equilibrated control sample).