Figure 3.
rsPMCA modeling of region-specific targeting was observed in Creutzfeldt-Jakob disease. A and D, amplification using vCJD#1/MM#1 (A) and sCJD/VV (D) seed/substrate couples are shown on Western blot illustrations. B and E, amplifications obtained with each seed/substrate couple are shown and in C and F have been plotted as a function of the PrPSc accumulation measured in the corresponding regions from the patient who provided the seed. Gand H, amplifications obtained with all human seed/substrate couples are plotted as a function of the PrPSc accumulation measured in the corresponding brain areas of the different patients who provided seeds (G), and corrected values for PrPC relative levels (H). Fro, frontal isocortex; Occ, occipital isocortex; Str, striatum; Pul, pulvinar; Cer, cerebellar cortex. Error bars represent S.D.; *, p < 0.05; **, p < 0.01. PMCA results are representative of three independent experiments performed in duplicates.