Fig. 6.

Decreased Cx43 and increased TLR4 expression in LPS stimulated human cardiac fibroblasts. Human cardiac fibroblasts were treated with LPS (10 ng/ml) for 24 h and unstimulated cells were used as controls. The cells were analyzed by western blotting with antibodies against Cx43, GLAST-1, GLT-1, Na⁺/K⁺ ATPase, TLR4 and β-actin. A) Immunoblotting pictures Relative intensities of protein bands were quantitated for. B) Cx43, C) GLAST-1, D) GLT-1, E) Na⁺/K⁺ ATPase and F) TLR4. Data are representative of four experiments and the intensities of control protein bands were set to 100%. Data are means ± SEM (n = 4). Student’s t-test, *P < 0.05, ***P < 0.001.