Fig 1. CBP1 alleles show a range of lytic abilities.
BMDMs were treated with 2.5 μg/mL tunicamycin (Tm), infected with indicated Hc strains at an MOI of 5, or mock infected (uninf). Macrophage lysis was measured by lactate dehydrogenase (LDH) release into culture supernatants and is presented as the percentage of total LDH in the supernatant and lysate of uninfected macrophages at 3 hpi. The lysis at each time point is an average of duplicate measurements of wells infected in triplicate, resulting in six total measurements, ± standard deviation. Macrophages treated with Tm or infected with wildtype Hc, cbp1+CBP1, cbp1+Pb_CBP1, or cbp1+cbp1D10A showed significantly more lysis compared to uninfected BMDMs at the indicated time points (**p<0.01, two-tailed Mann-Whitney test).