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. 2017 Sep 27;13(9):e1006589. doi: 10.1371/journal.ppat.1006589

Fig 3. Lytic CBP1 alleles activate the integrated stress response in infected macrophages.

Fig 3

BMDMs were treated with 2.5 μg/mL tunicamycin (Tm), infected with indicated Hc strains at an MOI of 5, or mock infected (uninf). Phosphorylated eIF2α (Ser51) (A) and ATF4 (B) were assessed by Western blot at 12 hpi. Representative blots are shown. The average signal intensity of phospho-eIF2α or ATF4 relative to the corresponding α-tubulin loading control from three replicates is shown in the bar graphs.*p<0.05, **p<0.01, compared to wildtype, two-tailed t-test. (C) Relative abundances of CHOP and TRIB3 transcripts were assessed by RT-qPCR at the indicated time points and normalized to uninfected BMDMs at 3 hpi.