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. 2017 Sep 27;13(9):e1006589. doi: 10.1371/journal.ppat.1006589

Fig 6. The host genes CHOP and TRIB3 are necessary for robust caspase-3/7 activity and macrophage death during Hc infection.

Fig 6

(A) Wildtype, CHOP-/-, and TRIB3-/- macrophages were mock infected or infected with wildtype Hc at an MOI of 5 or 10. Caspase-3/7 activity was measured 24 hpi and normalized to the activity in uninfected macrophages for each genotype. Each value is an average of triplicate wells ± standard deviation. *p<0.05, compared to wildtype, two-tailed t-test. (B) Wildtype, CHOP-/-, and TRIB3-/- BMDMs (MΦ) were infected with wildtype Hc at an MOI of 1 or mock infected (uninf), and macrophage lysis was measured by LDH release. BMDM lysis is presented as the percentage of total LDH in the supernatant and lysate of uninfected macrophages at 3 hpi. The lysis at each time point is an average of duplicate measurements of wells infected in triplicate, resulting in six total measurements, ± standard deviation.