Fig. 1.

Effects of chronic ethanol feeding on hepatic fat contents and the protein levels of Akt, GSK3β, SREBP-1c, and CYP2E1 in mice. Mice were exposed to control or ethanol-containing (5%, w/v) liquid diets for 1, 2, 3, and 4 weeks, respectively. (a) Histopathological examination was performed using Sudan Ⅲ and Oil Red O staining as described in the materials and methods section (Scale bar = 10 µm); (b) Serum TG levels; (c) hepatic TG levels; (d) Representative western blotting bands of p-Akt^ser473, p-Akt^thr308, Akt, p-GSK3β^ser9, GSK3β, mature form of SREBP-1c (n-SREBP-1c), and CYP2E1; (e)-(i) Quantitative data analyses of and p-Akt^thr308, p-Akt^ser473, p-GSK3β^ser9, and CYP2E1. Data were presented as mean ± SD (n = 5), and expressed as the percentage of the control.