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. 2017 Oct 3;10:4809–4819. doi: 10.2147/OTT.S140886

Figure 5.

Figure 5

Overexpression of HER2 rescues the migration and Rho activity of TNBC cells inhibited by kaempferol.

Notes: (A) MDA-MB-231 TNBC cells were transfected with HA-HER2 or vector, and then subjected to Western blot analysis. β-actin as the internal control. (B) MDA-MB-231 TNBC cells were transfected with HA-HER2 or vector, then allowed to migrate in response to 20 μmol/L kaempferol for 6 h. Results are presented as mean ± SD of 5 independent experiments. (C, D) MDA-MB-231 TNBC cells were transfected with HA-HER2 or vector, then incubated with 20 μmol/L kaempferol for 1 h, and subsequently subjected to the RhoA (C) and Rac1 (D) activity assays. The relative levels of Rho activity were normalized to the average value of controls. Results are presented as mean ± SD of 3 independent experiments. (E) MDA-MB-231 TNBC cells were transfected with HA-HER2 or vector, then allowed to invade in response to 20 μmol/L kaempferol for 6 h. Results are presented as mean ± SD of 3 independent experiments.

Abbreviations: Ctrl, control; HER2, human epidermal growth factor receptor 2; ns, no significance; TNBC, triple-negative breast cancer.