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Limit of Detection for VSV-G in Nuclease-free Water and Control Human DNA
(A) Quantification cycle (Cq) values for 10, 8, 6, 5, 4, and 3 copies/reaction and NTC. UND, undetectable; sample detection failed to reach the threshold of detection in 40 cycles (n = 4; 3 replicates per assay). (B) Number out of 3 replicates detectable within 40 reaction cycles for 10, 8, 6, 5, 4, and 3 copies/reaction and NTCs (n = 4). Error bars represent SD.
