Figure 4.

Limit of Detection for VSV-G in Control Human DNA with a 3% v/v Final Concentration of DMSO in the Reaction

(A) C_q values for 10, 8, 6, 5, 4, and 3 copies/reaction and NTC. UND, undetectable; sample detection failed to reach the threshold of detection in 40 cycles (n = 16; 3 replicates per assay). (B) Number of 3 replicates detectable within 40 reaction cycles for 10, 8, 6, 5, 4, and 3 copies/reaction and NTCs (n = 16). Data from Figure 2B are included for comparison. Error bars represent SD.