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. 2017 Aug 17;7(10):3251–3256. doi: 10.1534/g3.117.300166

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Copyright © 2017 Davis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Genome-wide RNAi screening methodology. (A) Outline of primary liquid RNAi screen. Approximately 10 L1 wild-type or nhl-2(0) worms were deposited into each well of a 96-well plate that contained bacteria expressing dsRNA clones. Black wells represent phenotypes that are present in wild-type animals with no enhancement in nhl-2(0) mutants. Gray wells represent positive hits, where enhanced phenotypes were observed in nhl-2(0) mutants. Positive hits were then validated by plate feeding RNAi (secondary screen). (B) Representative DIC images of phenotypes as observed during the scoring process (arrows).