Table 1. Genes from secondary screen listed per reported function.
| Gene (Phenotype) | Description (NCBI COGs) | Enriched | Homology | ||
|---|---|---|---|---|---|
| H | D | S | |||
| Transcriptional/nuclear organization hits | |||||
| mdt-8 (Lp) | Mediator of RNA polymerase II transcription subunit 8 | Sp | |||
| mdt-10 (Lp) | Mediator of RNA polymerase II transcription subunit 10 | Sp & Oo | |||
| mdt-27 (Lp) | Mediator of RNA polymerase II transcription subunit 27 | Sp & Oo | |||
| mrg-1 (Ste) | Chromodomain-containing protein | Sp | |||
| ntl-3 (Ste) | CCR4-NOT transcriptional regulation complex | ||||
| C36E8.1 (Ste) | RNA polymerase I transcription factor | Sp & Oo | |||
| spe-44 (Lp) | Nuclear DEAF-1 related transcriptional regulator | Sp & Oo | |||
| pqn-85 (Lp) | Sister chromatid cohesion protein | Sp & Oo | |||
| F58G1.2 (Lp) | Zn-finger protein | Sp | |||
| fem-1 (Lp) | Ankyrin repeat protein | Sp & Oo | |||
| Y54G2A.26 (Lp) | Unknown (transcriptional corepressor in S. pombe). | ||||
| sas-1 (Emb) | Spindle assembly protein | Sp | |||
| Cell cycle regulation | |||||
| cya-1 (Lp) | G2/mitotic-specific cyclin A | Sp & Oo | |||
| orc-5 (Emb) | Origin recognition complex | Sp & Oo | |||
| F35G12.11 (Lp) | Enhancer of rudimentary homolog | Sp | |||
| Motor related proteins | |||||
| arp-11(Emb) | Actin related protein | Sp & Oo | |||
| dli-1 (Emb) | Dynein light intermediate chain | Sp & Oo | |||
| dnc-1a (Lp) | Microtubule-associated protein | Sp & Oo | |||
| mop-25.2a (Lp) | Conserved mouse embryo scaffolding protein | Sp & Oo | |||
| F28D1.2 (Lp) | Myosin 1 homolog | Sp & Oo | |||
| Microtubule-related proteins | |||||
| dnc-1a (Lp) | Microtubule-associated protein | Sp & Oo | |||
| pfd-1 (Lp) | Molecular chaperone Prefoldin subunit 1 | ||||
| pfd-2 (Emb) | Molecular chaperone Prefoldin subunit 2 | Sp & Oo | |||
| Chromosomal integrity and DNA repair | |||||
| smz-2 (Emb) | Sperm meiosis PDZ domain containing protein | Sp | |||
| drh-3 (Emb) | DEAD-box RNA helicase | Sp & Oo | |||
| cde-1 (Emb) | Germline-specific nucleotidyltransferase protein | ||||
| ekl-1 a(Emb) | Tudor domain protein | ||||
| spdl-1 (Lp) | Coiled-coil protein | Sp & Oo | |||
| mop-25.2a (Lp) | Conserved mouse embryo scaffolding protein | Sp & Oo | |||
| rad-51 (Emb) | DNA repair protein | Sp & Oo | |||
| him-14(Emb) | MutS family of DNA mismatch repair protein | Sp & Oo | |||
| dsb-1 (Lp) | Double-strand break factor | Sp & Oo | |||
| Mitochondrial related proteins | |||||
| lpl-1 (Ste) | Lipoate ligase homolog | Sp | |||
| mtch-1 (Lp) | Mitochondrial carrier homolog 1 | Sp&Oo | |||
| Kinase proteins | |||||
| C56A3.8 (Lp) | Phosphatidylinositol 4-kinase type 2-alpha homolog | Sp & Oo | |||
| mpk-1 (Lp) | Mitogen-activated protein kinase | Sp | |||
| Other biological functions | |||||
| C06A8.6 (Lp) | Ortholog of human 1RRC9 | Sp & Oo | |||
| C34D4.4 (Lp) | Ortholog of human TVP23A | Sp & Oo | |||
| R05H5.3 (Emb) | Ortholog of human NXNl2 | Sp & Oo | |||
| unc-11 (Lp) | Clathrin assembly protein | Sp | |||
| T12A2.7 (Lp) | Ortholog of human BCAS2 | Oo | |||
| Proteins with unknown function | |||||
| C46A5.5 (Lp) | Unknown | Sp | |||
Phenotypes in nhl-2(ok818) mutants: Lp, low progeny; Ste, sterile; Emb, embryonic lethal. Gray = BLAST e value <0.1 and >30% query coverage. Black = BLAST e value <0.1 and >20% query coverage. White = BLAST e value >0.1. Sp = sperm enriched, Oo = oocyte enriched. Homology: H = H. sapiens, D = D. melanogaster, S = S. cerevisiae.
a
Genes with >1 reported function.