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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: Spine (Phila Pa 1976). 2017 Oct 15;42(20):1521–1528. doi: 10.1097/BRS.0000000000002258

Figure 1. Tobacco smoke upregulated disc cell ADAMTS5 protein expression.

Figure 1

(A) A representative Western blot showing the 73kDa cleaved form of ADAMTS5 protein (arrow) in the conditioned media of untreated (U) and treated hNP cell cultures with TSE for three days. M, protein markers. Right graph shows quantitation of the 73kDa ADAMTS5 band by densitometry (n=4). (B) Immunohistochemical detection of ADAMTS5 (brown stain, arrows) in the nucleus pulposus tissue section of unexposed (non-smokers) and exposed (smokers) WT mice. The bars represent 20 μm.