Table 1.
Protocol | 1st centrifugation | 2nd centrifugation | ||||
---|---|---|---|---|---|---|
Timing | Force (g) | Time (min) | Timing | Force (g) | Time (min) | |
A | Prefreeze | 800 | 10 | Not performed | N/A | N/A |
B | Prefreeze | 800 | 10 | Prefreeze | 11,000 | 1 |
C | Prefreeze | 800 | 10 | Post‐thaw | 11,000 | 1 |
D | Prefreeze | 380 | 20 | Prefreeze | 20,000 | 10 |
E | Prefreeze | 380 | 20 | Post‐thaw | 20,000 | 10 |