Figure 4.

Analysis of pirAB^vp-bearing plasmids pVCGX1 and pVPGX1. (A) Comparison of plasmids pVCGX1 and pVPGX1 with pVA1. Percentages indicate the identities in nucleotide level between syntenic regions. Percentages are not given for 100% identical regions. (B) A zoom-in view of the genetic context of pirAB^vp. Horizontal lines shown above the schematics, with both ends demarcated by solid triangles to indicated IRs, represent the ISVal1. Black block denotes the 5.5-kb pirAB^vp fragment describe in the text. (C) Putative evolution process of the genetic context of pirAB^vp on plasmid pVCGX1. Thin triangle indicates IS insertion. In the red block is the putative region going inversion. Nucleotide sequences flanking the ISVal1 elements are shown in 5′ → 3′. Sequences in identical colors are reverse-complement to each other as denoted in the black blocks. Gene organization is drawn to scale. Red arrows indicate pirAB^vp genes. Genes with other known functions are denoted using different colors. Syntenic regions were highlighted in gray.