FIGURE 4.

Corn infection with ham mutants. (A) 200 μl of 10⁶ spore/ml suspension of spores in 0.01% Tween 20 were inoculated on corn and compared to a mock control of 200 μl 0.01% Tween 20. The vials were kept in a moist chamber and placed in 29°C incubator with 12 h light/dark cycling and incubated for 5 days (Christensen et al., 2012). (B) The spores were washed off the seed with 3 ml 0.01% Tween 20 and counted. Each treatment was replicated three times. (C) Relative aflatoxin production of these mutants compared to WT (TXZ21.3.7). P-value, ^∗p < 0.05, ^∗∗p < 0.01.