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. 2017 Oct 5;8:1939. doi: 10.3389/fmicb.2017.01939

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Copyright © 2017 Chai, Qiao, Wang, Zhang, Wang, Wang, Zhou and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Polyphenol oxidase activity assay of PPO mutants and complementary strains on native-PAGE stained with L-DOPA. Gel lanes: (1) A. media strain WS (wild-type); (2) WS ΔyfiH; (3) WS ΔkatE; (4) WS ΔyfiHΔkatE, (5) WS ΔkatE complemented with an intact katE gene in trans on the plasmid pBBR1MCS-5.