Table 2.
Catalase activity assay.
| Samples | Protein concentration (μ g/mL) | Catalase activity |
|---|---|---|
| Cell supernatant of WS | nd | 0.12 ± 0.07 U/mL |
| Cell supernatant of WS ΔkatE | nd | 0.05 ± 0.04 U/mL |
| Intracellular extracts of WS | nd | 3.68 ± 0.28 U/mL |
| Intracellular extracts of WS ΔkatE | nd | 0.14 ± 0.09 U/mL |
| His6-tagged CatA | 288.34 ± 7.88 | 1,570.02 ± 46.75 U/mg |
| His6-tagged Hpi | 350.05 ± 3.24 | 463.82 ± 33.25 U/mg |
| His6-tagged Hpii | 329.74 ± 5.52 | 173.89 ± 20.86 U/mg |
nd, not determined: protein concentration was not measured for the catalase activity assay of A. media strain WS (wild-type) and the mutant strain WS ΔkatE.