Table 3.

Estimates of Araf content with different linkages in mg/g dry weight flour from integration of peaks in H1‐NMR spectra, assuming peak area per ¹H is the same as for the internal standard. Integrations were performed in regions A³‐X mono δ5.435–5.375, A³‐Xdi δ5.310–5.265, A²‐Xdi δ 5.255–5.210. For the region of A²‐Xdi peak in WE‐AX which overlaps with A‐AGP at around δ5.255 (Figure 6), area was estimated using values from the other side of the peak as the peak is symmetrical. Mean values are shown ± SEM, n = 3. H/A is ratio of transgenic mean to azygous control mean as %, with P‐value from Student's t‐test for H‐A comparison shown in parentheses

Fraction	RNAi target	Trans‐gene	A3‐Xmono		A3‐Xdi		A2‐Xdi
			Mean	H/A	Mean	H/A	Mean	H/A
WE	TaGT43_2	A	0.37 ± 0.03	30% (0.002)	0.21 ± 0.01	62% (0.002)	0.23 ± 0.01	51% (0.000)
		H	0.11 ± 0.01		0.13 ± 0.01		0.12 ± 0.00
	TaGT47_2	A	0.32 ± 0.03	30% (0.004)	0.18 ± 0.02	63% (0.014)	0.19 ± 0.00	57% (0.000)
		H	0.10 ± 0.01		0.12 ± 0.00		0.11 ± 0.01
	TaXAT1	A	0.47 ± 0.02	29% (0.000)	0.24 ± 0.00	69% (0.000)	0.28 ± 0.00	69% (0.000)
		H	0.14 ± 0.01		0.17 ± 0.00		0.19 ± 0.00
XE	TaGT43_2	A	4.21 ± 0.11	51% (0.000)	1.52 ± 0.04	53% (0.000)	1.84 ± 0.04	67% (0.000)
		H	2.15 ± 0.05		0.80 ± 0.01		1.23 ± 0.01
	TaGT47_2	A	4.27 ± 0.10	58% (0.000)	1.47 ± 0.02	64% (0.000)	1.85 ± 0.03	72% (0.002)
		H	2.46 ± 0.12		0.94 ± 0.03		1.34 ± 0.07
	TaXAT1	A	3.84 ± 0.12	73% (0.002)	1.25 ± 0.04	141% (0.000)	1.60 ± 0.05	130% (0.001)
		H	2.80 ± 0.05		1.76 ± 0.01		2.07 ± 0.04